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    Peptide Calculator for Reconstitution & Dosage

    Enter your peptide vial size in mg and desired dose, and FoxAI automatically generates the ideal BAC water volume and exact dosage to draw in syringe units. Supports GLP-1s, KLOW & GLOW cocktails, BPC-157, NAD+ and 30+ peptides.

    FoxAI peptide reconstitution and dosage calculator

    FoxAI will help you calculate ideal BAC water volume (mL) and exact syringe units. You will need:

    • Peptide Vial Size (mg)
    • Desired Peptide Dose (mg or mcg)

    1. Select Compound

    2. Vial Size

    mg

    3. Desired Concentration

    mg/mL

    4. Desired Dose

    per injection (mg)

    5. Instructions

    Syringe Units to Draw
    —units
    Volume to Inject
    —mL
    • •Use sterile bacteriostatic water (0.9% benzyl alcohol)
    • •Add diluent slowly down vial wall to minimize foaming
    • •Swirl gently—do NOT shake vigorously
    • •Solution should be clear and colorless; discard if cloudy or discolored

    How do I calculate peptide reconstitution (BAC water) and dosage (syringe units)?

    To calculate dosage for any peptide, GLP-1 (e.g., tirzepatide), or supporting compound such as NAD+, divide the total peptide in the vial (mg) by the amount of bacteriostatic (BAC) water added (mL) to find the concentration, then divide the desired dose by this concentration to determine the injection volume.

    Peptide Reconstitution and Dosage Calculation Steps

    1. Start with Vial Size: Identify total milligrams (mg) on the vial (e.g., 2 mg, 5 mg, 10 mg).
    2. Add BAC Water: Add a precise amount of BAC water, typically 1 mL or 2 mL, to the vial to dissolve the peptide.

    3. Determine Concentration (example: 10 mg vial ÷ 1 mL water = 10 mg/mL):

      Concentration (mg/mL)=Total BAC Water (mL)Total Peptide (mg)​

    4. Calculate Dosage Volume (example: 1 mg dose ÷ 10 mg/mL = 0.1 mL):

      Volume to Draw (mL)=Concentration (mg/mL)Desired Dose (mg)​

    5. Convert to Syringe Units: Using a standard U-100 (1 mL/100 unit) insulin syringe, 0.1 mL equals 10 units.

    Standard Reconstitution Examples (Targeting 10–50 Units)

    • 250 mcg (0.25 mg) dose in a 10 mg vial: Add 1 mL BAC water = 25 units (0.25 mL draw).
    • 500 mcg (0.5 mg) dose in a 10 mg vial: Add 2 mL BAC water = 25 units (0.25 mL draw).
    • 1 mg dose in a 10 mg vial: Add 2 mL BAC water = 20 units (0.2 mL draw).
    • 2.5 mg dose in a 20 mg vial: Add 2 mL BAC water = 25 units (0.25 mL draw).
    • 4 mg dose in a 20 mg vial: Add 2 mL BAC water = 40 units (0.4 mL draw).

    Key Considerations

    • Units vs. ML: 100 units on a syringe equals 1 mL.
    • Precision: Use smaller syringes (e.g., 30-unit or 50-unit) for more accurate, smaller doses.
    • Reconstitution: Add water slowly down the side of the vial, then gently swirl to dissolve; do not shake.
    • Accuracy: Use the FoxAI peptide calculator to automatically double-check calculations and conversions.
    Updated April 19, 2026

    General Reconstitution & Math

    What is the formula for calculating peptide injection volume?

    Volume (mL) = Desired dose (mg) ÷ Concentration (mg/mL)

    For example, if you added 2 mL of BAC water to a 5 mg peptide vial, your concentration is 2.5 mg/mL. A 0.25 mg dose = 0.25 ÷ 2.5 = 0.1 mL = 10 units on an insulin syringe.

    The calculator does this arithmetic for you — just enter your vial mg, water volume, and desired dose.

    How do I convert mg to mcg for peptide dosing?

    Multiply milligrams (mg) by 1,000 to get micrograms (mcg). For example, 2 mg is equal to 2,000 mcg.

    Most healing peptides (BPC-157, TB-500, GHK-Cu) are dosed in mcg (250–500 mcg).

    What syringe should I use for peptide injections?

    Insulin syringes (0.5 mL or 1 mL, 29–31 gauge) are standard for subcutaneous peptide injections. They're marked in units where 100 units = 1 mL. This calculator shows both unit and mL measurements.

    What happens if I add too much bacteriostatic water?

    Nothing is ruined — you've just created a lower concentration, which means a larger injection volume to hit your target dose.

    Update the BAC water field in the calculator and it will recalculate automatically. The peptide is unaffected — dilution changes the volume per dose, not the compound.

    How do I convert peptide milligrams (mg) to insulin syringe units?
    1. Calculate your peptide vial's concentration in mg/mL.Concentration (mg/mL)=BAC Water (mL)Vial Size (mg)​Example: a 10 mg vial reconstituted with 2 mL of BAC water yields 5 mg/mL.
    2. Convert your dose into syringe units. On a U-100 syringe, 1 mL = 100 units.Syringe Units=Concentration (mg/mL)Desired Dose (mg)​×100Example: a 0.5 mg dose at 5 mg/mL = (0.5 ÷ 5) × 100 = 10 units.

    Standard Insulin Syringe Capacities

    • 0.3 mL Syringe: 30 units max — ideal for small, precise doses under 30 units.
    • 0.5 mL Syringe: 50 units max — ideal for standard 25–50 unit doses.
    • 1.0 mL Syringe: 100 units max — ideal for large reconstitution volumes or doses over 50 units.
    • 3.0 mL Syringe: 300 units max — ideal for larger intramuscular injections for NAD+, Glutathione, and other support compounds.

    3 mL syringes are also used for reconstitution to avoid multiple transfers between BAC water and peptide vials.

    How much BAC water should I use for less common doses or non-standard vial sizes?

    When a dose doesn't divide cleanly into the total milligrams in the vial, adjust the water volume to force the math. Adding a precise, uneven amount of water (like 2.4 mL) ensures your daily injection always lands on a clean 10- or 50-unit mark, minimizing dosing errors.

    Steps to Force Clean Syringe Math

    1. Set Target Syringe Units: Lock your desired draw to a round number, such as 10 units (0.1 mL), 25 units (0.25 mL) or 50 units (0.5 mL).
    2. Apply the Reconstitution Formula: Multiply the vial size by the target volume, then divide by the desired dose.
    Ideal BAC Water (mL)=Desired Dose (mg)Vial Size (mg)×Target Volume (mL)​

    Examples for Tricky Reconstitutions

    • 400 mcg (0.4 mg) dose in a 10 mg vial: Target 10 units (0.1 mL). Add 2.5 mL BAC water.
    • 2.2 mg dose from a 20 mg vial: Target 25 units (0.25 mL). Add 2.27 mL BAC water.
    • 750 mcg (0.75 mg) dose in a 10 mg vial: Target 20 units (0.2 mL). Add 2.66 mL BAC water.
    • 2.5 mg dose in a 12 mg vial: Target 50 units (0.5 mL). Add 2.4 mL BAC water.
    • 5 mg dose in a 24 mg vial: Target 50 units (0.5 mL). Add 2.4 mL BAC water.

    GLP-1 Reconstitution

    How much BAC water do I add to my retatrutide vial?

    Each vial size has a target dose range. Within that range, doses with the same optimal recipe share a row; doses needing different BAC water get their own. For each vial size: BAC water → working concentration → dose = units (volume).

    5 mg vial

    • 3 mL BAC water → 1.67 mg/mL: 0.5 mg = 30 units (0.3 mL); 1.5 mg = 90 units (0.9 mL).
    • 2.5 mL BAC water → 2 mg/mL: 1 mg = 50 units (0.5 mL); 2 mg = 100 units (1 mL — full syringe).
    • 1 mL BAC water → 5 mg/mL: 2.5 mg = 50 units (0.5 mL).

    10 mg vial

    • 3 mL BAC water → 3.33 mg/mL: 0.5 mg = 15 units (0.15 mL); 1 mg = 30 units (0.3 mL); 2.5 mg = 75 units (0.75 mL).
    • 2.5 mL BAC water → 4 mg/mL: 2 mg = 50 units (0.5 mL); 4 mg = 100 units (1 mL — full syringe).
    • 1 mL BAC water → 10 mg/mL: 5 mg = 50 units (0.5 mL).

    12 mg vial

    • 3 mL BAC water → 4 mg/mL: 1 mg = 25 units (0.25 mL); 2 mg = 50 units (0.5 mL); 4 mg = 100 units (1 mL — full syringe).
    • 1 mL BAC water → 12 mg/mL: 6 mg = 50 units (0.5 mL).

    20 mg vial

    • 3 mL BAC water → 6.67 mg/mL: 1 mg = 15 units (0.15 mL); 2 mg = 30 units (0.3 mL); 6 mg = 90 units (0.9 mL).
    • 2.5 mL BAC water → 8 mg/mL: 4 mg = 50 units (0.5 mL); 8 mg = 100 units (1 mL — full syringe).
    • 1 mL BAC water → 20 mg/mL: 6 mg = 30 units (0.3 mL); 8 mg = 40 units (0.4 mL); 12 mg = 60 units (0.6 mL).

    24 mg vial

    • 3 mL BAC water → 8 mg/mL: 4 mg = 50 units (0.5 mL); 6 mg = 75 units (0.75 mL); 8 mg = 100 units (1 mL — full syringe).
    • 1 mL BAC water → 24 mg/mL: 12 mg = 50 units (0.5 mL).

    30 mg vial

    • 2.3 mL BAC water → 13.04 mg/mL: 4 mg = 30 units (0.3 mL).
    • 2.5 mL BAC water → 12 mg/mL: 6 mg = 50 units (0.5 mL); 12 mg = 100 units (1 mL — full syringe).
    • 3 mL BAC water → 10 mg/mL: 8 mg = 80 units (0.8 mL).
    How much BAC water do I add to my tirzepatide vial?

    Each vial size has a target dose range. Within that range, doses with the same optimal recipe share a row; doses needing different BAC water get their own. For each vial size: BAC water → working concentration → dose = units (volume).

    10 mg vial

    • 3 mL BAC water → 3.33 mg/mL: 2.5 mg = 75 units (0.75 mL).
    • 1 mL BAC water → 10 mg/mL: 5 mg = 50 units (0.5 mL).

    15 mg vial

    • 3 mL BAC water → 5 mg/mL: 2.5 mg = 50 units (0.5 mL); 5 mg = 100 units (1 mL — full syringe).
    • 1 mL BAC water → 15 mg/mL: 7.5 mg = 50 units (0.5 mL).

    20 mg vial

    • 2.4 mL BAC water → 8.33 mg/mL: 2.5 mg = 30 units (0.3 mL); 7.5 mg = 90 units (0.9 mL).
    • 3 mL BAC water → 6.67 mg/mL: 5 mg = 75 units (0.75 mL).
    • 1 mL BAC water → 20 mg/mL: 10 mg = 50 units (0.5 mL).

    30 mg vial

    • 3 mL BAC water → 10 mg/mL: 5 mg = 50 units (0.5 mL); 7.5 mg = 75 units (0.75 mL); 10 mg = 100 units (1 mL — full syringe).
    • 2.4 mL BAC water → 12.5 mg/mL: 12.5 mg = 100 units (1 mL — full syringe).
    • 1 mL BAC water → 30 mg/mL: 15 mg = 50 units (0.5 mL).

    40 mg vial

    • 2.7 mL BAC water → 14.81 mg/mL: 7.5 mg = 50 units (0.5 mL).
    • 3 mL BAC water → 13.33 mg/mL: 10 mg = 75 units (0.75 mL).
    • 2.4 mL BAC water → 16.67 mg/mL: 12.5 mg = 75 units (0.75 mL); 15 mg = 90 units (0.9 mL).

    50 mg vial

    • 2.7 mL BAC water → 18.52 mg/mL: 7.5 mg = 40 units (0.4 mL).
    • 2.5 mL BAC water → 20 mg/mL: 10 mg = 50 units (0.5 mL).
    • 3 mL BAC water → 16.67 mg/mL: 12.5 mg = 75 units (0.75 mL); 15 mg = 90 units (0.9 mL).

    60 mg vial

    • 2.4 mL BAC water → 25 mg/mL: 7.5 mg = 30 units (0.3 mL); 12.5 mg = 50 units (0.5 mL).
    • 3 mL BAC water → 20 mg/mL: 10 mg = 50 units (0.5 mL); 15 mg = 75 units (0.75 mL).
    How much BAC water do I add to my semaglutide vial?

    Each vial size has a target dose range. Within that range, doses with the same optimal recipe share a row; doses needing different BAC water get their own. For each vial size: BAC water → working concentration → dose = units (volume).

    2.5 mg vial

    • 3 mL BAC water → 0.83 mg/mL: 0.25 mg = 30 units (0.3 mL).
    • 2.5 mL BAC water → 1 mg/mL: 0.5 mg = 50 units (0.5 mL); 1 mg = 100 units (1 mL — full syringe).

    5 mg vial

    • 3 mL BAC water → 1.67 mg/mL: 0.25 mg = 15 units (0.15 mL); 0.5 mg = 30 units (0.3 mL).
    • 2.5 mL BAC water → 2 mg/mL: 1 mg = 50 units (0.5 mL); 2 mg = 100 units (1 mL — full syringe).

    10 mg vial

    • 3 mL BAC water → 3.33 mg/mL: 0.5 mg = 15 units (0.15 mL); 1 mg = 30 units (0.3 mL).
    • 2.5 mL BAC water → 4 mg/mL: 2 mg = 50 units (0.5 mL).
    • 2.1 mL BAC water → 4.76 mg/mL: 2.4 mg = 50 units (0.5 mL).

    Retatrutide, Semaglutide, & Tirzepatide Dosing by Vial Size

    How do I reconstitute a 10 mg retatrutide vial?

    The recipe depends on your target dose. Use the reconstitution formula below to compute BAC water for the dose you're targeting.

    1. Calculate your BAC water volume. The reconstitution formula:BAC Water (mL)=Desired Dose (mg)Vial Size (mg)×Target Draw (mL)​
      • Example 1 (1 mg dose, target 30 units / 0.3 mL): 10 × 0.3 ÷ 1 = 3 mL BAC water → 3.33 mg/mL.
      • Example 2 (5 mg dose, target 50 units / 0.5 mL): 10 × 0.5 ÷ 5 = 1 mL BAC water → 10 mg/mL.
    2. Sanitize. Wash your hands, put on gloves, and wipe the rubber stoppers of both the retatrutide vial and the bacteriostatic water vial with an alcohol swab.
    3. Draw BAC water. Draw air equal to your calculated volume, invert the BAC water vial, inject the air to equalize pressure, and slowly draw out the required volume.
    4. Inject slowly. Angle the needle toward the inside glass wall of the retatrutide vial and inject slowly — never forcefully onto the powder.
    5. Dissolve. Swirl gently until completely clear. Do not shake — agitation damages the peptide.
    6. Store. Refrigerate immediately (36–46°F) and use within 4–6 weeks.
    How do I reconstitute a 20 mg retatrutide vial?

    The recipe depends on your target dose. Use the reconstitution formula below to compute BAC water for the dose you're targeting.

    1. Calculate your BAC water volume. The reconstitution formula: ext{BAC Water (mL)} = rac{ ext{Vial Size (mg)} imes ext{Target Draw (mL)}}{ ext{Desired Dose (mg)}}
      • Example 1 (2 mg dose, target 30 units / 0.3 mL): 20 × 0.3 ÷ 2 = 3 mL BAC water → 6.67 mg/mL.
      • Example 2 (12 mg dose, target 60 units / 0.6 mL): 20 × 0.6 ÷ 12 = 1 mL BAC water → 20 mg/mL.
    2. Sanitize. Wash your hands, put on gloves, and wipe the rubber stoppers of both the retatrutide vial and the bacteriostatic water vial with an alcohol swab.
    3. Draw BAC water. Draw air equal to your calculated volume, invert the BAC water vial, inject the air to equalize pressure, and slowly draw out the required volume.
    4. Inject slowly. Angle the needle toward the inside glass wall of the retatrutide vial and inject slowly — never forcefully onto the powder.
    5. Dissolve. Swirl gently until completely clear. Do not shake — agitation damages the peptide.
    6. Store. Refrigerate immediately (36–46°F) and use within 4–6 weeks.
    How do I reconstitute a 10 mg tirzepatide vial?

    The recipe depends on your target dose. Use the reconstitution formula below to compute BAC water for the dose you're targeting.

    1. Calculate your BAC water volume. The reconstitution formula:BAC Water (mL)=Desired Dose (mg)Vial Size (mg)×Target Draw (mL)​
      • Example 1 (2.5 mg dose, target 75 units / 0.75 mL): 10 × 0.75 ÷ 2.5 = 3 mL BAC water → 3.33 mg/mL.
      • Example 2 (5 mg dose, target 50 units / 0.5 mL): 10 × 0.5 ÷ 5 = 1 mL BAC water → 10 mg/mL.
    2. Sanitize. Wash your hands, put on gloves, and wipe the rubber stoppers of both the tirzepatide vial and the bacteriostatic water vial with an alcohol swab.
    3. Draw BAC water. Draw air equal to your calculated volume, invert the BAC water vial, inject the air to equalize pressure, and slowly draw out the required volume.
    4. Inject slowly. Angle the needle toward the inside glass wall of the tirzepatide vial and inject slowly — never forcefully onto the powder.
    5. Dissolve. Swirl gently until completely clear. Do not shake — agitation damages the peptide.
    6. Store. Refrigerate immediately (36–46°F) and use within 4–6 weeks.
    How do I reconstitute a 30 mg tirzepatide vial?

    The recipe depends on your target dose. Use the reconstitution formula below to compute BAC water for the dose you're targeting.

    1. Calculate your BAC water volume. The reconstitution formula:BAC Water (mL)=Desired Dose (mg)Vial Size (mg)×Target Draw (mL)​
      • Example 1 (7.5 mg dose, target 75 units / 0.75 mL): 30 × 0.75 ÷ 7.5 = 3 mL BAC water → 10 mg/mL.
      • Example 2 (15 mg dose, target 50 units / 0.5 mL): 30 × 0.5 ÷ 15 = 1 mL BAC water → 30 mg/mL.
    2. Sanitize. Wash your hands, put on gloves, and wipe the rubber stoppers of both the tirzepatide vial and the bacteriostatic water vial with an alcohol swab.
    3. Draw BAC water. Draw air equal to your calculated volume, invert the BAC water vial, inject the air to equalize pressure, and slowly draw out the required volume.
    4. Inject slowly. Angle the needle toward the inside glass wall of the tirzepatide vial and inject slowly — never forcefully onto the powder.
    5. Dissolve. Swirl gently until completely clear. Do not shake — agitation damages the peptide.
    6. Store. Refrigerate immediately (36–46°F) and use within 4–6 weeks.
    How do I reconstitute a 5 mg semaglutide vial?

    The recipe depends on your target dose. Use the reconstitution formula below to compute BAC water for the dose you're targeting.

    1. Calculate your BAC water volume. The reconstitution formula:BAC Water (mL)=Desired Dose (mg)Vial Size (mg)×Target Draw (mL)​
      • Example 1 (0.25 mg dose, target 15 units / 0.15 mL): 5 × 0.15 ÷ 0.25 = 3 mL BAC water → 1.67 mg/mL.
      • Example 2 (1 mg dose, target 50 units / 0.5 mL): 5 × 0.5 ÷ 1 = 2.5 mL BAC water → 2 mg/mL.
    2. Sanitize. Wash your hands, put on gloves, and wipe the rubber stoppers of both the semaglutide vial and the bacteriostatic water vial with an alcohol swab.
    3. Draw BAC water. Draw air equal to your calculated volume, invert the BAC water vial, inject the air to equalize pressure, and slowly draw out the required volume.
    4. Inject slowly. Angle the needle toward the inside glass wall of the semaglutide vial and inject slowly — never forcefully onto the powder.
    5. Dissolve. Swirl gently until completely clear. Do not shake — agitation damages the peptide.
    6. Store. Refrigerate immediately (36–46°F) and use within 4–6 weeks.
    How do I reconstitute a 10 mg semaglutide vial?

    The recipe depends on your target dose. Use the reconstitution formula below to compute BAC water for the dose you're targeting.

    1. Calculate your BAC water volume. The reconstitution formula:BAC Water (mL)=Desired Dose (mg)Vial Size (mg)×Target Draw (mL)​
      • Example 1 (0.5 mg dose, target 15 units / 0.15 mL): 10 × 0.15 ÷ 0.5 = 3 mL BAC water → 3.33 mg/mL.
      • Example 2 (2 mg dose, target 50 units / 0.5 mL): 10 × 0.5 ÷ 2 = 2.5 mL BAC water → 4 mg/mL.
    2. Sanitize. Wash your hands, put on gloves, and wipe the rubber stoppers of both the semaglutide vial and the bacteriostatic water vial with an alcohol swab.
    3. Draw BAC water. Draw air equal to your calculated volume, invert the BAC water vial, inject the air to equalize pressure, and slowly draw out the required volume.
    4. Inject slowly. Angle the needle toward the inside glass wall of the semaglutide vial and inject slowly — never forcefully onto the powder.
    5. Dissolve. Swirl gently until completely clear. Do not shake — agitation damages the peptide.
    6. Store. Refrigerate immediately (36–46°F) and use within 4–6 weeks.

    KLOW, GLOW, and Multi-Peptide Blend Reconstitution

    How do I calculate dosage and BAC water volume for a KLOW 80 mg peptide blend?
    1. Confirm your vial size. KLOW is typically 80 mg total: 10 mg BPC-157, 10 mg TB-4, 10 mg KPV, 50 mg GHK-Cu.
    2. Pick an anchor compound. Anchor your dose to whichever compound matches your goal — GHK-Cu for skincare, BPC-157 for injury repair — and the other three peptides come along at fixed proportions.
    3. Pick a BAC water volume that lands on a clean insulin-syringe mark. Two defaults that both land on 10 units (0.1 mL) per dose on a U-100 insulin syringe:
      • Skincare — 2 mg GHK-Cu anchor, reconstitute the 80 mg vial with 2.5 mL BAC water.
      • Injury — 0.5 mg BPC-157 anchor, reconstitute the 80 mg vial with 2 mL BAC water.
    4. Solve for your per-dose draw volume.Vdraw​=Manchor​Danchor​×Vwater​​

      Danchor​ is your target dose in mg, Vwater​ is the BAC water volume you reconstitute with in mL, and Manchor​ is the anchor compound mass in the vial in mg.

    5. Plug in the Step 3 defaults to see what each dose delivers.
      • Skincare (2.5 mL water with GHK-Cu anchor) — Payload: GHK-Cu 2 mg · BPC-157 0.4 mg · TB-4 0.4 mg · KPV 0.4 mg.Vdraw​=50mg2mg×2.5mL​=0.1mL=10units
      • Injury (2 mL water with BPC-157 anchor) — Payload: GHK-Cu 2.5 mg · BPC-157 0.5 mg · TB-4 0.5 mg · KPV 0.5 mg.Vdraw​=10mg0.5mg×2mL​=0.1mL=10units

    Larger vials or injection irritation: double the water volume and double your draw — delivered dose is identical.

    How do I calculate dosage and BAC water volume for a GLOW 70 mg peptide blend?
    1. Confirm your vial size. GLOW is typically 70 mg total: 10 mg BPC-157, 10 mg TB-4, 50 mg GHK-Cu.
    2. Pick an anchor compound. Anchor your dose to whichever compound matches your goal — GHK-Cu for skincare, BPC-157 for injury repair — and the other two peptides come along at fixed proportions.
    3. Pick a BAC water volume that lands on a clean insulin-syringe mark. Two defaults that both land on 10 units (0.1 mL) per dose on a U-100 insulin syringe:
      • Skincare — 2 mg GHK-Cu anchor, reconstitute the 70 mg vial with 2.5 mL BAC water.
      • Injury — 0.5 mg BPC-157 anchor, reconstitute the 70 mg vial with 2 mL BAC water.
    4. Solve for your per-dose draw volume.Vdraw​=Manchor​Danchor​×Vwater​​

      Danchor​ is your target dose in mg, Vwater​ is the BAC water volume you reconstitute with in mL, and Manchor​ is the anchor compound mass in the vial in mg.

    5. Plug in the Step 3 defaults to see what each dose delivers.
      • Skincare (2.5 mL water with GHK-Cu anchor) — Payload: GHK-Cu 2 mg · BPC-157 0.4 mg · TB-4 0.4 mg.Vdraw​=50mg2mg×2.5mL​=0.1mL=10units
      • Injury (2 mL water with BPC-157 anchor) — Payload: GHK-Cu 2.5 mg · BPC-157 0.5 mg · TB-4 0.5 mg.Vdraw​=10mg0.5mg×2mL​=0.1mL=10units

    Larger vials or injection irritation: double the water volume and double your draw — delivered dose is identical.

    How do I calculate dosage and BAC water volume for the Wolverine Stack (BPC-157 / TB-500) blend?
    1. Confirm your vial composition. Standard blend is BPC-157 10 mg + TB-500 10 mg = 20 mg total, 1:1 ratio. Equal mass means one calculation covers both peptides simultaneously.
    2. Pick a BAC water volume that lands on a clean insulin-syringe mark. Reconstitute the 20 mg blend vial with 2 mL BAC water. At a standard 500 mcg per-compound dose, this lands on 10 units (0.1 mL) on a U-100 insulin syringe.
    3. Solve for your per-dose draw volume.Vdraw​=MD×Vwater​​

      D is your target dose per compound in mg, Vwater​ is the BAC water volume you reconstitute with in mL, and M is the single-compound mass in the vial in mg.

    4. Plug in the Step 2 default to see what each dose delivers. At 2 mL BAC water and 500 mcg per compound:Vdraw​=10mg0.5mg×2mL​=0.1mL=10unitsPayload at 10 units: BPC-157 500 mcg · TB-500 500 mcg.

    Wolverine Stack Dosing Profile (20 mg Blend / 2 mL BAC Water)

    • 250 mcg each: Draw 5 units (0.05 mL)
    • 500 mcg each: Draw 10 units (0.1 mL) — standard BPC-157 dose
    • 1 mg each: Draw 20 units (0.2 mL)
    • 2 mg each: Draw 40 units (0.4 mL) — weekly TB-500 loading range

    Other Peptides

    How do I calculate dosage and BAC water volume for MOTS-c?

    MOTS-c triggers the same cellular response as an endurance session — shifting metabolism toward fat oxidation. A 5 mg to 10 mg dose injected prior to exercise is standard for maximizing this metabolic adaptation.

    Reconstitution Note: Use 0.9% NaCl Bacteriostatic Water, not plain BAC water. MOTS-c frequently causes injection-site welts; sodium chloride dampens this localized skin reaction. Use the reconstituted vial within 1–2 weeks.

    1. Reconstitute for clean math. Add 1 mL of 0.9% NaCl bacteriostatic water to the 10 mg vial.Concentration=BAC Water (mL)Total Peptide (mg)​=1mL10mg​=10mg/mL
    2. Calculate your draw volume. For a 5 mg target dose:Volume to Draw=ConcentrationDesired Dose​=10mg/mL5mg​=0.5mL
    3. Convert to syringe units. On a U-100 insulin syringe, 1 mL = 100 units.Syringe Units=Volume to Draw (mL)×100=0.5mL×100=50units

    MOTS-c Dosing Profile (10 mg Vial / 1 mL BAC Water)

    • 5 mg Dose: Draw 50 units (0.5 mL)
    • 10 mg Dose: Draw 100 units (1.0 mL)
    How do I calculate dosage and BAC water volume for NAD+?

    NAD+ drives cellular energy and mitochondrial function. At 50 mg to 250 mg per dose, the high injection volume requires intramuscular (IM) administration or splitting the dose across multiple subcutaneous sites.

    Reconstitution Note: Reconstitute NAD+ with 0.9% NaCl Bacteriostatic Water to prevent injection-site pain from acidity and osmotic mismatch. For optimal pain management, source NAD+ pre-buffered with sodium bicarbonate.

    1. Reconstitute for clean math. Choose your vial size — each lands at the same working concentration:
      • 250 mg vial: Add 2.5 mL → 100 mg/mL
      • 500 mg vial: Add 5 mL → 100 mg/mL
      • 1,000 mg vial: Add 5 mL → 200 mg/mL
      Concentration=BAC Water (mL)Total Peptide (mg)​=2.5mL250mg​=100mg/mL
    2. Calculate your draw volume. For a 100 mg target dose at 100 mg/mL:Volume to Draw=ConcentrationDesired Dose​=100mg/mL100mg​=1.0mL
    3. Convert to syringe units. On a U-100 insulin syringe, 1 mL = 100 units.Syringe Units=Volume to Draw (mL)×100=1.0mL×100=100units

    NAD+ Dosing Profile (100 mg/mL Concentration)

    • 50 mg Dose: Draw 50 units (0.5 mL)
    • 100 mg Dose: Draw 100 units (1.0 mL)
    • 150 mg Dose: Draw 150 units (1.5 mL) — Requires two syringes or a larger 3 mL IM syringe.
    How do I calculate dosage and BAC water volume for BPC-157?

    BPC-157 accelerates healing by directing blood vessel cells to sprout new capillaries into damaged tissue. Patients typically target 250 mcg to 500 mcg injected subcutaneously to support tendon recovery and gut inflammation.

    1. Reconstitute for clean math. Add 2 mL of bacteriostatic water to the 10 mg vial.Concentration=BAC Water (mL)Total Peptide (mg)​=2mL10mg​=5mg/mL
    2. Calculate your draw volume. For a 500 mcg (0.5 mg) target dose:Volume to Draw=ConcentrationDesired Dose​=5mg/mL0.5mg​=0.1mL
    3. Convert to syringe units. On a U-100 insulin syringe, 1 mL = 100 units.Syringe Units=Volume to Draw (mL)×100=0.1mL×100=10units

    BPC-157 Dosing Profile (10 mg Vial / 2 mL BAC Water)

    • 250 mcg Dose: Draw 5 units (0.05 mL)
    • 500 mcg Dose: Draw 10 units (0.1 mL)
    How do I calculate dosage and BAC water volume for TB-500?

    TB-500 is a synthetic fraction of Thymosin Beta-4, explicitly favored for acute muscle and tissue repair. Rather than daily injections, the protocol requires a heavier 2.5 mg systemic dose injected twice per week.

    1. Reconstitute for clean math. Add 1 mL of bacteriostatic water to the 10 mg vial.Concentration=BAC Water (mL)Total Peptide (mg)​=1mL10mg​=10mg/mL
    2. Calculate your draw volume. For a 2.5 mg target dose:Volume to Draw=ConcentrationDesired Dose​=10mg/mL2.5mg​=0.25mL
    3. Convert to syringe units. On a U-100 insulin syringe, 1 mL = 100 units.Syringe Units=Volume to Draw (mL)×100=0.25mL×100=25units

    TB-500 Dosing Profile (10 mg Vial / 1 mL BAC Water)

    • 2.5 mg Dose: Draw 25 units (0.25 mL)
    • 5 mg Dose: Draw 50 units (0.5 mL)
    How do I calculate dosage and BAC water volume for Semax?

    Semax supports cognitive function and focus by enhancing brain-derived neurotrophic factor (BDNF). It is dosed between 300 mcg and 1,000 mcg (1 mg) daily, either subcutaneously or via an intranasal applicator.

    1. Reconstitute for clean math. Add 2 mL of bacteriostatic water to the 10 mg vial.Concentration=BAC Water (mL)Total Peptide (mg)​=2mL10mg​=5mg/mL
    2. Calculate your draw volume. For a 1 mg (1,000 mcg) target dose:Volume to Draw=ConcentrationDesired Dose​=5mg/mL1mg​=0.2mL
    3. Convert to syringe units. On a U-100 insulin syringe, 1 mL = 100 units.Syringe Units=Volume to Draw (mL)×100=0.2mL×100=20units

    Semax Dosing Profile (10 mg Vial / 2 mL BAC Water)

    • 300 mcg Dose: Draw 6 units (0.06 mL)
    • 1 mg (1,000 mcg) Dose: Draw 20 units (0.2 mL)

    Medical Disclaimer

    The content in this calculator is for informational purposes only and does not constitute medical advice. Always consult with a qualified healthcare provider before beginning any new protocol, supplement, or medication.

    FoxAIPeptide BAC Water and Dosing Calculator
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